Human tear proteome dataset in response to daily wear of water gradient contact lens using SWATH-MS approach.

Water Gradient Contact Lens (WGCL) is a new generation material that combines the benefits of Silicone hydrogel (SiHy) and traditional hydrogel contact lenses by modifying the materials between the core and the surface. However, its impact on tear proteome has not been explored. Tears were collected on healthy young adults using Schirmer's strip at baseline, 1-week, and 1-month of WGCL lens wear (n=15) and age-matched untouched controls (n=10). Equal amounts of tears samples from individuals of WGCL and control groups were randomly pooled to form representative equal parts at each condition (n=3 for WGCL wear and age-matched untouched control group) at each condition (baseline, 1-week, and 1-month). Tears were prepared using the S-Trap sample preparation followed by the analysis of a TripleTOF 6600 mass spectrometer. Using Information-dependent acquisition (IDA), a total of 725 tear proteins (6760 distinct peptides) were identified in the constructed spectral library at 1% FDR. Using data-independent acquisition (SWATH-MS), data were analyzed and processed using PeakView (v2.2, SCIEX), with the top differentially expressed proteins at each time point (baseline, 1-week, and 1-month) presented. All acquired raw data (IDA and SWATH-MS) were submitted and published on the Peptide Atlas public repository (http://www.peptideatlas.org/) for general release (Data ID PASS01589).

Robust effects of predictability across experimental contexts: Evidence from event-related potentials.

Many experiments have shown that comprehenders can generate predictions about upcoming inputs on the fly, but relatively little is known about whether and how comprehenders' sensitivity to predictability may be modulated by the experimental context. The present study used event-related potentials (ERPs) in two experiments to ask whether changing the overall predictive validity of the stimuli will affect comprehenders' brain responses to predictable as well as unpredictable words by manipulating the filler sentences, which made up 50% of the stimuli in each experiment. Contrary to the prediction that predictable words should be processed more easily and elicit a smaller N400 response in a more prediction-encouraging experimental context, we found that participants' N400 response to predictable as well as unpredictable words was smaller when the overall predictive validity of the stimuli was low (that is, when the filler items were incongruous compared to when they were predictable). Further, even though the use of different filler sentences did modulate comprehenders' ERP and behavioural responses, it did not modulate the effect of target word predictability on participants' ERP responses at all. We take the present findings to suggest that healthy young adults' brain responses are inherently sensitive to the predictability of the incoming linguistic stimuli, and that this robust sensitivity can be observed regardless of the make-up of the experimental stimuli.

Eye-tracking evidence for active gap-filling regardless of dependency length.

Previous work on real-time sentence processing has established that comprehenders build and interpret filler-gap dependencies without waiting for unambiguous evidence about the actual location of the gap ("active gap-filling") as long as such dependencies are grammatically licensed. However, this generalisation was called into question by recent findings in a self-paced reading experiment by Wagers and Phillips, which may be taken to show that comprehenders do not interpret the filler at the posited gap when the dependency spans a longer distance. In the present study, we aimed to replicate these findings in an eye-tracking experiment with better controlled materials and increased statistical power. Crucially, we found clear evidence for active gap-filling across all levels of dependency length. This diverges from Wagers and Phillips's findings but is in line with the long-standing generalisation that comprehenders build and interpret filler-gap dependencies predictively as long as they are grammatically licensed. We found that the effect became smaller in the long dependency conditions in the post-critical region, which suggests the weaker effect in the long dependency conditions may have been undetected in Wagers and Phillips's study due to insufficient statistical power and/or the use of a self-paced reading paradigm.

Effects of subject-case marking on agreement processing: ERP evidence from Basque.

Previous cross-linguistic research has found that comprehenders are immediately sensitive to various kinds of agreement violations across languages. We focused on Basque, a verb-final ergative language with both subject-verb (SV) and object-verb (OV) agreement. We compared the effects of SV agreement violations on comprehenders' event-related brain potentials (ERPs) in transitive sentences (where OV agreement is present, and the subject is ergative) and intransitive sentences (where OV agreement is absent, and the subject is absolutive). We observed a P600 effect in both cases, but only violations with intransitive subjects elicited an early posterior negativity. Such a qualitative difference suggests that distinct neurocognitive mechanisms are involved in processing agreement with transitive subjects (which are marked with ergative case) versus intransitive subjects (which bear absolutive case). Building on theoretical proposals that in languages such as Basque, true agreement occurs with absolutive subjects but not with ergative subjects, we submit that the early posterior negativity may be an electrophysiological signature for true agreement.

Coreference and antecedent representation across languages.

Previous studies have shown that speakers of languages such as German, Spanish, and French reactivate the syntactic gender of the antecedent of a pronoun to license gender agreement. As syntactic gender is assumed to be stored in the lexicon, this has motivated the claim that pronouns in these languages reactivate the lexical entry of their antecedent noun. In contrast, in languages without syntactic gender such as English, lexical retrieval might be unnecessary. We used eye-tracking while reading to examine whether antecedent retrieval involves rapid semantic and phonological reactivation. We compared German and English. In German, we found early sensitivity to the semantic but not to the phonological features of the pronoun's antecedent. In English, readers did not immediately show either semantic or phonological effects specific to coreference. We propose that early semantic facilitation arises due to syntactic gender reactivation, and that antecedent retrieval varies cross-linguistically depending on the type of information relevant to the grammar of each language. (PsycINFO Database Record

Development of online use of theory of mind during adolescence: An eye-tracking study.

We investigated the development of theory of mind use through eye-tracking in children (9-13years old, n=14), adolescents (14-17.9years old, n=28), and adults (19-29years old, n=23). Participants performed a computerized task in which a director instructed them to move objects placed on a set of shelves. Some of the objects were blocked off from the director's point of view; therefore, participants needed to take into consideration the director's ignorance of these objects when following the director's instructions. In a control condition, participants performed the same task in the absence of the director and were told that the instructions would refer only to items in slots without a back panel, controlling for general cognitive demands of the task. Participants also performed two inhibitory control tasks. We replicated previous findings, namely that in the director-present condition, but not in the control condition, children and adolescents made more errors than adults, suggesting that theory of mind use improves between adolescence and adulthood. Inhibitory control partly accounted for errors on the director task, indicating that it is a factor of developmental change in perspective taking. Eye-tracking data revealed early eye gaze differences between trials where the director's perspective was taken into account and those where it was not. Once differences in accuracy rates were considered, all age groups engaged in the same kind of online processing during perspective taking but differed in how often they engaged in perspective taking. When perspective is correctly taken, all age groups' gaze data point to an early influence of perspective information.

The Relationship Between Anaphor Features and Antecedent Retrieval: Comparing Mandarin Ziji and Ta-Ziji.

In the present study we report two self-paced reading experiments that investigate antecedent retrieval processes in sentence comprehension by contrasting the real-time processing behavior of two different reflexive anaphors in Mandarin Chinese. Previous work has suggested that comprehenders initially evaluate the fit between the morphologically simple long-distance reflexive "ziji" and the closest available subject position, only subsequently considering more structurally distant antecedents (Gao et al., 2005; Liu, 2009; Li and Zhou, 2010; Dillon et al., 2014; cf. Chen et al., 2012). In this paper, we investigate whether this locality bias effect obtains for other reflexive anaphors in Mandarin Chinese, or if it is associated specifically with the morphologically simple reflexive ziji. We do this by comparing the processing of ziji to the processing of the morphologically complex reflexive ta-ziji (lit. s/he-self). In Experiment 1, we investigate the processing of ziji, and replicate the finding of a strong locality bias effect for ziji in self-paced reading measures. In Experiment 2, we investigate the processing of the morphologically complex reflexive ta-ziji in the same structural configurations as Experiment 1. A comparison of our experiments reveals that ta-ziji shows a significantly weaker locality bias effect than ziji does. We propose that this results from the difference in the number of morphological and semantic features on the anaphor ta-ziji relative to ziji. Specifically, we propose that the additional retrieval cues associated with ta-ziji reduce interference from irrelevant representations in memory, allowing it to more reliably access an antecedent regardless its linear or structural distance. This reduced interference in turn leads to a diminished locality bias effect for the morphologically complex anaphor ta-ziji.

The structure-sensitivity of memory access: evidence from Mandarin Chinese.

The present study examined the processing of the Mandarin Chinese long-distance reflexive ziji to evaluate the role that syntactic structure plays in the memory retrieval operations that support sentence comprehension. Using the multiple-response speed-accuracy tradeoff (MR-SAT) paradigm, we measured the speed with which comprehenders retrieve an antecedent for ziji. Our experimental materials contrasted sentences where ziji's antecedent was in the local clause with sentences where ziji's antecedent was in a distant clause. Time course results from MR-SAT suggest that ziji dependencies with syntactically distant antecedents are slower to process than syntactically local dependencies. To aid in interpreting the SAT data, we present a formal model of the antecedent retrieval process, and derive quantitative predictions about the time course of antecedent retrieval. The modeling results support the Local Search hypothesis: during syntactic retrieval, comprehenders initially limit memory search to the local syntactic domain. We argue that Local Search hypothesis has important implications for theories of locality effects in sentence comprehension. In particular, our results suggest that not all locality effects may be reduced to the effects of temporal decay and retrieval interference.

Immediate sensitivity to structural constraints in pronoun resolution.

Real-time interpretation of pronouns is sometimes sensitive to the presence of grammatically-illicit antecedents and sometimes not. This occasional sensitivity has been taken as evidence that structural constraints do not immediately impact the initial antecedent retrieval for pronoun interpretation. We argue that it is important to separate effects that reflect the initial antecedent retrieval process from those that reflect later processes. We present results from five reading comprehension experiments. Both the current results and previous evidence support the hypothesis that agreement features and structural constraints immediately constrain the antecedent retrieval process for pronoun interpretation. Occasional sensitivity to grammatically-illicit antecedents may be due to repair processes triggered when the initial retrieval fails to return a grammatical antecedent.

Additive effects of repetition and predictability during comprehension: evidence from event-related potentials.

Previous research has shown that neural responses to words during sentence comprehension are sensitive to both lexical repetition and a word's predictability in context. While previous research has often contrasted the effects of these variables (e.g. by looking at cases in which word repetition violates sentence-level constraints), little is known about how they work in tandem. In the current study we examine how recent exposure to a word and its predictability in context combine to impact lexical semantic processing. We devise a novel paradigm that combines reading comprehension with a recognition memory task, allowing for an orthogonal manipulation of a word's predictability and its repetition status. Using event-related brain potentials (ERPs), we show that word repetition and predictability have qualitatively similar and additive effects on the N400 amplitude. We propose that prior exposure to a word and predictability impact lexical semantic processing in an additive and independent fashion.

No semantic illusions in the "Semantic P600" phenomenon: ERP evidence from Mandarin Chinese.

Recent observations of unexpected ERP responses to grammatically well-formed role-reversed sentences (the "Semantic P600" phenomenon) have been taken to bear directly on questions about the architecture of the language processing system. This paper evaluates two central pieces of evidence for accounts that propose a syntax-independent semantic composition mechanism, namely the presence of P600 effects and the absence of N400 effects in role-reversed sentences. Experiment 1 examined the relative contribution of the presence of an animacy violation and the semantic relations between words ('combinability') to the ERP responses to role-reversed sentences. Experiment 2 examined the ERP responses to role-reversed sentences that are fully animacy-congruous. Results from the two experiments showed that animacy-violated sentences with no plausible non-surface interpretation elicited the same P600 effect as both types of role-reversed sentences; additionally, semantically anomalous target words elicited no N400 effects when they were strongly semantically related to the preceding words, regardless of the presence of animacy violations. Taken together, these findings suggest that the presence of P600s to role-reversed sentences can be attributed to the implausibility of the sentence meaning, and the absence of N400 effects is due to a combination of weak contextual constraints and strong lexical association. The presence of a plausible non-surface interpretation and animacy violations made no unique contribution to the ERP response profiles. Hence, existing ERP findings are compatible with the long-held assumption that online semantic composition is dependent on surface syntax and do not constitute evidence for a syntax-independent semantic composition mechanism.